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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 31, No. 1, 2018, pp. 44-47
Bioline Code: ej18006
Full paper language: English
Document type: Short Communication
Document available free of charge

Electronic Journal of Biotechnology, Vol. 31, No. 1, 2018, pp. 44-47

 en Development and characterization of InDel markers for Lupinus luteus check for this species in other resources L. (Fabaceae) and cross-species amplification in other Lupin species
Osorio, Claudia E.; Udall, Joshua A.; Salvo-Garrido, Haroldo & Maureira-Butler, Iván J.

Abstract

Background: Strong artificial selection and/or natural bottle necks may limit genetic variation in domesticated species. Lupinus luteus, an orphan temperate crop, has suffered diversity reductions during its bitter/sweet alkaloid domestication history, limiting breeding efforts and making molecular marker development a difficult task. The main goal of this research was to generate new polymorphic insertion–deletion (InDel) markers to aid yellow lupin genetics and breeding. By combining genomic reduction libraries and next generation sequencing, several polymorphic InDel markers were developed for L. luteus L.
Results: A total of 118 InDel in silico polymorphic markers were identified. Eighteen InDel primer sets were evaluated in a diverse L. luteus core collection, where amplified between 2–3 alleles per locus. Observed heterozygosity (HO; 0.0648 to 0.5564) and polymorphic information content (PIC; 0.06 to 0.48) estimations revealed a moderate level of genetic variation across L. luteus accessions. In addition, ten and nine InDel loci amplified successfully Lupinus hispanicus check for this species in other resources Boiss & Reut, and Lupinus mutabilis check for this species in other resources Sweet, respectively, two L. luteus close relatives. PCA analysis identified two L. luteus clusters, most likely explained by the domestication species history.
Conclusion: The development of InDel markers will facilitate the study of genetic diversity across L. luteus populations, as well as among closely related species.

Keywords
Accession; Genetic diversity; Genomic reduction libraries; In silico polymorphic markers; Insertion–deletion marker; Lupinus hispanicus; Lupinus mutabilis; Next generation sequencing; Plant domestication

 
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