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Electronic Journal of Biotechnology
Universidad Católica de Valparaíso
ISSN: 0717-3458
Vol. 31, No. 1, 2018, pp. 67-74
Bioline Code: ej18010
Full paper language: English
Document type: Research Article
Document available free of charge

Electronic Journal of Biotechnology, Vol. 31, No. 1, 2018, pp. 67-74

 en Chemical pretreatment of Arundo donax check for this species in other resources L. for second-generation ethanol production
Lemões, Juliana Silva; Silva, Claudia Fernanda Lemons e; Avila, Sabrina Peres Farias; Montero, Cândida Raquel Scherrer; Silva, Sérgio Delmar dos Anjos e; Samios, Dimitrios & Peralba, Maria do Carmo Ruaro


Background: Pretreatment of lignocellulosic biomass is essential for using it as a raw material for chemical and biofuel production. This study evaluates the effects of variables in the chemical pretreatment of the Arundo biomass on the glucose and xylose concentrations in the final enzymatic hydrolysate. Three pretreatments were tested: acid pretreatment, acid pretreatment followed by alkaline pretreatment, and alkaline pretreatment.
Results: The amounts of glucose and xylose released by the enzymatic hydrolysis of the Arundo biomass obtained from acid pretreatment ranged from 6.2 to 19.1 g/L and 1.8 to 3.1 g/L, respectively. The addition of alkaline pretreatment led to a higher yield from the enzymatic hydrolysis, with the average glucose concentration 3.5 times that obtained after biomass hydrolysis with an acid pretreatment exclusively. The use of an alkaline pretreatment alone resulted in glucose and xylose concentrations similar to those obtained in the two-step pretreatment: acid pretreatment followed by alkaline pretreatment. There was no significant difference in 5-hydroxymethylfurfural, furfural, or acetic acid concentrations among the pretreatments.
Conclusion: Alkaline pretreatment was essential for obtaining high concentrations of glucose and xylose. The application of an alkaline pretreatment alone resulted in high glucose and xylose concentrations. This result is very significant as it allows a cost reduction by eliminating one step.

Acid pretreatment; Alkaline pretreatment; Arundo biomass; Biofuel; Enzymatic hydrolysis; Giant reed; Glucose; Lignocellulose; Response surface methodology; Xylose; Raw material

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