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Indian Journal of Human Genetics
Medknow Publications on behalf of Indian Society of Human Genetics
ISSN: 0971-6866
EISSN: 0971-6866
Vol. 11, No. 1, 2005, pp. 35-38
Bioline Code: hg05005
Full paper language: English
Document type: Research Article
Document available free of charge

Indian Journal of Human Genetics, Vol. 11, No. 1, 2005, pp. 35-38

 en Detection of Helicobactor pylori by polymerase chain reaction: A comparison in sample preparation
Murugesan R., Bhattacharjee P., Vivek Kumar P.R., Mohankumar Mary N., Nagarajan Mangala, Jeevanram R.K.


Gastric biopsy samples obtained from 14 patients with upper abdominal pain, clinically diagnosed as acid peptic disease, were analysed for the presence of Helicobacter pylori (H. pylori) by Polymerase Chain Reaction (PCR) using partially (template A) and completely purified DNA (template B). Antigen specific primer was used to analyse the sample by PCR method. The presence of H. pylori in the samples was confirmed by running a positive control. The presence of H. pylori was also detected by urease method using standard protocol. Among the 14 samples studied, 8 showed the presence of H. pylori with both templates A and B. Among these 8 samples only 3 showed positive for the presence of H. pylori with urease method. The present work discusses the results obtained in the detection of H. pylori in template A and B by PCR method.

H. pylori , Gastritis, Duodenal ulcer, Polymerase Chain Reaction, Antigen specific primer

© Copyright 2005 Indian Journal of Human Genetics.
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