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Recombination in human leukocyte antigen region in two Asian Indian families Mishra, Mahendra N. & Sharma, Ajay Abstract Background: Recombination (crossing over) may generate novel haplotypes that can be beneficial to a population against recently introduced pathogens. It may lead to the generation of new alleles. Settings and Design: A prospective study at a tertiary care centre. Aim : To report two rare cases of crossing over in HLA region. Materials and Methods: Tissue-typing was done by sequence specific primers (SSP) for DR locus and by both SSP and serology for Class I which was reconfirmed on fresh samples. Results: In one patient crossing over had taken place in the region of A locus resulting in inheritance of AFNx0101 instead of expected A*11. In second family crossing over had taken place in region of DRB1 locus and the sibling inherited DRB1*08 instead of DRB1*10. Conclusions: Possibility of recombination must be considered when interpreting implausible tissue-typing results of families worked up for BMT. Keywords AML, human leukocyte antigen, recombination, sequence specific primers

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