The study evaluated the antibiotic resistance patterns of Helicobacter pylori
strains against metronidazole and
clarithromycin in a hospital in Havana, Cuba. Eighty-five percent, 22.5%, and 10% of 40 H. pylori
investigated were resistant to metronidazole, ciprofloxacin, and clarithromycin respectively but all were
susceptible to amoxicillin and tetracycline. RdxA truncation was found only in metronidazole-resistant
strains. In such strains, reported are eight and two novel mutations in the rdxA and frxA genes respectively.
Two-point mutations in the 23S rRNA
genes of clarithromycin-resistant strains were detected. A high prevalence
of metronidazole resistance was found in Cuban H. pylori
strains. Mutations in the rdxA
contribute more significantly than frxA gene
to the high level of resistance to metronidazole. This study
supports the need to continue monitoring the antibiotic susceptibility in H. pylori
in Cuba to guide the
treatment of such infection.