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The Journal of Health, Population and Nutrition
icddr,b
ISSN: 1606-0997
EISSN: 2072-1315
Vol. 35, No. 1, 2016, pp. 1-9
Bioline Code: hn16019
Full paper language: English
Document type: Research Article
Document available free of charge

The Journal of Health, Population and Nutrition, Vol. 35, No. 1, 2016, pp. 1-9

 en Identification of bacterial contaminants in polyherbal medicines used for the treatment of tuberculosis in Amatole District of the Eastern Cape Province, South Africa, using rapid 16S rRNA technique
Famewo, Elizabeth Bosede; Clarke, Anna Maria & Afolayan, Anthony Jide

Abstract

Background: Polyherbal medicines are used for the treatment of many diseases in many African and Asian communities. With the increasing use of these remedies, several investigations have shown that they are associated with a broad variety of residues and contaminants. This study investigates the presence of bacteria in the polyherbal medicines used for the treatment of tuberculosis (TB) in the Eastern Cape Province of South Africa.
Methods: Bacterial DNA was extracted from the polyherbal medicines, and a fragment of the bacterial 16S rRNA gene was amplified by PCR with universal primers 27F and 518R. The amplicons were visualised on agarose gel electrophoresis, followed by end repair and adaptor ligation. They were further purified and quantified using Library Preparation kit NEBNext® UltraT DNA Library Prep Kit for Illumina, and the amplicons were run on illumina’s MiSeq platform.
Results: Different bacterial species were identified in all each of the polyherbal medicines. Generally, the most prominent and common bacteria recovered from all the samples were Bacillus check for this species in other resources sp., Enterobacter check for this species in other resources sp., Klebsiella check for this species in other resources sp., Rahnella check for this species in other resources sp., Paenibacillus check for this species in other resources sp., Clostridium check for this species in other resources sp. and Pantoea check for this species in other resources sp. Others are Pseudomonas check for this species in other resources sp., Raoultella ornithinolytica check for this species in other resources , Salmonella enterica check for this species in other resources and Eubacterium moniliforme check for this species in other resources .
Conclusions: This study, thus, revealed the presence of pathogenic and non-pathogenic bacteria in the polyherbal medicines used for the treatment of tuberculosis in the study area. The implications of the findings are discussed in relation to the health care of the patients of tuberculosis in the study area, having in mind that they are immunocompromised individuals.

Keywords
Polyherbal medicines; Tuberculosis; Bacteria; Public health; Sequencing

 
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