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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 1, No. 1, 2001, pp. 3-8
Bioline Code: hs01004
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 1, No. 1, 2001, pp. 3-8

 en Evaluation of the Adjuvant Effect of Escherichia coli check for this species in other resources Heat-labile Enterotoxin Mutant (LTK63) on the Systemic Immune Responses to Intranasally Co-administered Measles Virus Nucleoprotein. Part I: Antibody Responses
Erume, Joseph & Partidos, Harris


The adjuvanticity and immunogenicity of the heat-labile enterotoxin (LT) of Escherichia coli check for this species in other resources and of its non-toxic mutant, LTK63, was evaluated after intranasal administration of CBA mice with recombinant measles virus nucleoprotein (rMVNP) with or without LT or LTK63. Both LT and LTK63 were shown to be highly immunogenic with higher responses observed 4 weeks after the booster immunization. Although the nucleoprotein was immunogenic on its own, mice immunized with the nucleoprotein plus wild type LT produced significantly high antibody responses (p<0.01). Mice that received the rMVNP with LTK63 also generated strong antibody responses to rMVNP. These antibodies were also significantly higher than those of rMVNP alone (p< 0.05). No significant differences were observed between groups of mice immunized intranasally with rMVNP plus LT or LTK63 (p> 0.05). Data on IgG antibody isotype profiles showed that IgG 1 and IgG 2a were predominant in mice immunized with rMVNP + LT or LTK63 whereas IgG 1 predominated when rMVNP was given on its own implying that LT and LTK63 induce both Th1 and Th2-type immune responses. These results highlight the great potential of this non-toxic mutant of LT as a safe vaccine adjuvant.

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