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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 13, No. 3, 2013, pp. 551-555
Bioline Code: hs13084
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 13, No. 3, 2013, pp. 551-555

 en Probe substrate and enzyme source-dependent inhibition of UDPglucuronosyltransferase (UGT) 1A9 by wogonin
Chengcheng, G; Rui, Xie; Tianheng, Ma; Wei, Yan & Liqun, Pang


Background: Drug-metabolizing enzymes (DMEs) inhibition based drug-drug interaction and herb-drug interaction severely challenge the R&D process of drugs or herbal ingredients.
Objective: To evaluate the inhibition potential of wogonin (an important flavonoid isolated from the root of Scutellaria baicalensis check for this species in other resources ) towards one of the most important phase II DMEs, UDP-glucuronosyltransferase (UGT) 1A9.
Methods: Both recombinant UGT1A9-catalyzed 4-methylumbelliferone (4-MU) glucuronidation reaction and human liver microsomes (HLMs)-catalyzed propofol glucuronidation reaction were used as two different probe reactions.
Results: Wogonin noncompetitively inhibited recombinant UGT1A9-catalyzed 4-MU glucuronidation, and exerted competitive inhibition towards HLMs-catalyzed propofol glucuronidation. The inhibition kinetic parameters (Ki) were calculated to be 3.2 μM and 52.0μM, respectively.
Conclusion: Necessary monitoring was needed when wogonin was co-administered with the clinical drugs mainly undergoing UGT1A9-mediated glucuronidation elimination. Additionally, probe reactions-dependent inhibition of wogonin towards the activity of UGT1A9 should be paid attention when translating these in vitro data into in vivo situation.

wogonin; drug-drug interaction (DDI); UDP-glucuronosyltransferases (UGTs)

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