Effect of Ginkgo biloba extract on the expressions of Cox-2 and GST-Pi in rats with hepatocellular carcinoma risk|
Chao, Ou; Hai-Ping, Zheng; Jian-Jia, Su; Ji, Cao; Guo-Jian, Li & Le-Qun, Li
Background:Hepatocellular carcinoma (HCC) is one of the most common and aggressive cancers worldwide, and the
pathogenesis is complicated at present. There iare few effective therapeutic measures, and novel therapeutic strategies are
urgently required to improve clinical outcome. Ginkgo biloba extract (EGb) is reported to have an anti-cancer activity.
Objectives: To explore the effect of EGb on expressions of cyclooxygenase-2 (Cox-2) and glutathione S-transferase Pi
(GST-Pi) in the pathogenesis of HCC.
Methods: 120 Wistar rats were divided into three groups at random: normal control group (control group), HCC risk group
without treatment (HCC risk group), HCC risk group treated with EGb (EGb group); n=40, respectively. The HCC risk in
rat was induced by aflatoxin B1 injection. At the end of 13-week, 33-week, 53-week and 73-week, 10 rats in each group were
killed and the relevant samples were collected.
Results:The mRNA and protein expressions of Cox-2 and GST-Pi were measured by real-time reverse transcription
polymerase chain reaction, immunohistochemical analysis and western-blot. When compared with those in the control
group in 73-week, the mRNA and protein expressions of GST-Pi in EGb group were weaker than those in HCC risk group
in 73-week. However, the mRNA and protein expressions of Cox-2 in HCC risk group were increased than that of control
group, and there was no statistical difference for mRNA and protein expressions of Cox-2 between HCC risk group and
Conclusion: EGb can regulate the expression of GST-Pi, but it does not seem to have an effect on Cox-2 expression in the
liver of HCC risk rats.
Hepatocellular carcinoma (HCC); Ginkgo biloba extract (EGb); Cox-2; GST-Pi