In this study, we attempted to screen and investigate antibacterial activity of Bacillus
species, which were isolated from conjunctiva, against other eyes pathogens.
To examine predominant isolates of Bacillus subtilis
, B. pumilus
, B. cereus
and B. mojevensis
, isolated from conjunctiva for their antimicrobial activity against indicator microorganisms as Micrococcus luteus
, Staphyloccocus aureus
, S. epidermidis
, S. hominis
, S. lugdunensis
, S. warneri
, S. haemolyticus
, B. cereus
, Listeria monocytogenes
, and Proteus mirabilis
. Growth inhibitions of indicator microorganisms were tested using agar diffusion tests by cells and supernatants of five B. mojevensis
, one B. subtilis
, four B. cereus
and five B. pumilus
strains which were isolated from conjunctiva.
isolates showed variable ability of inhibition against the tested microorganisms. Two strains of B. pumillus
, 1 strain of B. subtilis
, 5 strains of B. mojevensis
, 1 strain of B. cereus
were efficacious against the tested microorganisms. Most resistant microorganism to these bacteria was Proteus mirabilis
. Two of Gram positive bacteria, S. lugdenensis
(K15-9) and S. aureus
(SDA48), were also found as resistant.
In this study, Bacillus
spp isolated from conjunctiva showed antimicrobial activity against Gram-positive bacteria. Human eye-derived microorganisms and their antimicrobial effects might be a useful source of natural products for the future.