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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 15, No. 1, 2015, pp. 97-100
Bioline Code: hs15014
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 15, No. 1, 2015, pp. 97-100

 en Inhibitory effect of emodin on human hepatoma cell line SMMC-7721 and its mechanism
Zhang, Xia; Chen, Yingping; Zhang, Ting & Zhang, Yaming

Abstract

Background: Da Huang (Radix et Rhizoma Rhei) is the dried root or rhizome of Rheum palmatum check for this species in other resources L., Rheum tanguticum check for this species in other resources Maxim ex Balf. or Rheum officinale check for this species in other resources Braill of family Polygonaceae. It has heat clearing, damp drying, fire purging and toxin removing effects. Because of its definite curative efficacy, it has been widely applied in clinical settings.
Objective: To study the inhibitory effect of emodin on human hepatoma cell line SMMC-7721 and its mechanism.
Methods: MTT assay, flow cytometry and electron microscopy were used to investigate the inhibitory effect of different concentrations of emodin on human hepatoma cell line SMMC-7721.
Results: 12 h, 24 h and 48 h after the action of 20, 40 and 80 umol/L emodin on SMMC-7721 cells, the proliferation of human hepatoma SMMC-7721 cells was inhibited; the inhibitory effects showed time-and concentration-dependence. 48 h after the action of different concentrations of emodin on SMMC-7721 cells, cells in G2/M phase increased significantly, while the proportion of S phase cells gradually declined.
Conclusion: Emodin can inhibit human hepatoma cell line SMMC-7721.

Keywords
emodin; SMMC-7721; MTT assay

 
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