African Health Sciences
Makerere University Medical School
Vol. 15, No. 2, 2015, pp. 360-367
Bioline Code: hs15054
Full paper language: English
Document type: Research Article
Document available free of charge
African Health Sciences, Vol. 15, No. 2, 2015, pp. 360-367
© Copyright 2015 - African Health Sciences
Evaluation of the diagnostic performance and operational characteristics of four rapid immunochromatographic syphilis tests in Burkina Faso|
Bocoum, Fadima Yaya; Ouédraogo, Henri; Tarnagda, Grissoum; Kiba, Alice; Tiendrebeogo, Simon; Bationo, Fabrice; Liestman, Benjamin; Diagbouga, Serge; Zarowsky, Christina; Traoré, Ramata Ouédraogo & Kouanda, Séni
Background and Objective: Little information is available on the rapid diagnostic testing for syphilis in Burkina Faso. The
objectives of the study were (i) to assess the sensitivity and specificity of four on site rapid tests in comparison with Treponema
pallidum haemagglutination assay (TPHA) as a gold standard and (ii) to evaluate the operational characteristics of
those tests among health workers in a maternity unit.
Methods: Four rapid syphilis tests commercially available in Burkina Faso were evaluated using archived serum samples and
Treponema pallidum hemagglutination assay (TPHA) as the gold standard. Blood samples were collected between November
2011 and June 2012 from blood donors at the Regional Blood Transfusion Center of Ouagadougou. The sensitivity and
specificity of the tests were calculated. Evaluation of operational characteristics such as clarity of pamphlet, complexity of
technique, duration, was conducted in a first-level healthcare center with health workers in maternity unit.
Results: Alere DetermineTM Syphilis was the most sensitive of the four rapid syphilis tests evaluated. It was followed by SD
Bioline Syphilis 3.0, Cypress Diagnostics Syphilis Quick test and Accu-Tell ® Rapid Anti-TP, which was the least sensitive.
The four tests demonstrated a good diagnostic specificity for syphilis (95–98%), and healthcare workers found them easy
Conclusions: The study allowed confirming the good performance of three of four rapid syphilis tests in Burkina Faso.
More research will be conducted to assess the feasibility of introducing selected rapid tests for syphilis in antenatal care
syphilis; rapid test; performance; Burkina Faso