African Health Sciences
Makerere University Medical School
Vol. 15, No. 2, 2015, pp. 641-646
Bioline Code: hs15089
Full paper language: English
Document type: Research Article
Document available free of charge
African Health Sciences, Vol. 15, No. 2, 2015, pp. 641-646
© Copyright 2015 - African Health Sciences
Comparison of a fluorometric assay kit with high-performance liquid chromatography for the assessment of serum retinol concentration.|
Elom, Aglago Kouassivi; Imane, El Menchawy; Kaoutar, Benjeddou; Khalid, El Kari; Asmaa, El Hamdouchi; Mehdi, Azlaf; Noureddine, El Haloui & Hassan, Aguenaou
Background: Although high-performance liquid chromatography (HPLC) is the commonly used method for the analysis
of retinol in biological samples, simple and rapid test kits are available.
Objectives: This study compared a rapid test kit (ICHECK Fluoro®) to HPLC for the assessment of serum retinol concentrations.
Methods: For the analysis by HPLC, sample preparation included standard deproteinization and extraction phases. The
analysis by ICHECK was performed by injecting serum into IEX reagent vials (n=89) and mixing manually for separation.
After precipitation of the proteins, the vial was introduced into the chamber of the ICHECK Fluoro and analysed at 0 min
(ICHECK0min) and 15 min later (ICHECK15min). Bland and Altman approach was applied to test the agreement between
HPLC and ICHECK.
Results: Mean HPLC, ICHECK0min and ICHECK15min values were 421.2±106.0 μg/L, 423.1±118.3 μg/L and
413.2±107.6 μg/L, respectively. Retinol concentrations significantly decreased in the IEX solution over time (p<0.001). No
significant proportional bias was observed between HPLC and ICHECK0min (r-0.038, p=0.73) and ICHECK15min (r=-
0.024, p=0.82). Fixed biases (HPLC minus ICHECK) for ICHECK0min and ICHECK15min were respectively -1.9±23.1
μg/l (p=0.45) and 8.0±22.7 μg/l (p=0.002).
Conclusion: ICHECK Fluoro may offer a reliable mean for assessing serum retinol for measurements performed with no
significant time delay.
HPLC; ICHECK Fluoro; serum retinol; test kit; vitamin A status