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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 15, No. 3, 2015, pp. 748-754
Bioline Code: hs15104
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 15, No. 3, 2015, pp. 748-754

 en Polymerase chain reaction to search for Herpes viruses in uveitic and healthy eyes: a South African perspective
Laaks, Debbie; Smit, Derrick P. & Harvey, Justin


Objective: To analyse aqueous polymerase chain reaction (PCR) results in patients diagnosed with undifferentiated uveitis and determine prevalence of herpesviridae in non-uveitic patients undergoing routine cataract extraction.
Design: Retrospective comparative case series and prospective cross-sectional study.
Subjects: 72 patients with idiopathic uveitis and 57 surgical patients.
Methods: Diagnostic aqueous paracentesis with PCR testing for 6 herpes viridae in uveitic patients. Anterior chamber paracentesis immediately pre-operative in the prospective arm, with PCR testing.
Results: In the retrospective review we had a 47.2% positive PCR yield. Data analysis revealed a statistically significant correlation between a positive yield and being HIV+ (p=0.018); between an EBV+ yield and being HIV+ (p= 0.026) and a CMV+ result and being HIV+ (p=0.032). Posterior uveitis (p=0.014) and symptoms <30 days (p= 0.0014) had a statistically significant yield. In the prospective arm of the study: all 57 patients were HIV- and all aqueous samples were negative for the 6 herpesviridae.
Conclusion: We recommend PCR testing for Herpesviridae as a safe second line test for patients with undifferentiated uveitis. We were unable to establish prevalence and suggest that the idea of a commensal herpes virus is unlikely if the blood-ocular barrier is intact.

Polymerase chain reaction, Herpes viruses, uveitis, healthy eyes, South Africa

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