African Health Sciences
Makerere University Medical School
Vol. 15, No. 3, 2015, pp. 789-796
Bioline Code: hs15109
Full paper language: English
Document type: Research Article
Document available free of charge
African Health Sciences, Vol. 15, No. 3, 2015, pp. 789-796
© Copyright 2015 - African Health Sciences
Evaluation of the anti-candidal activity of methanolic leaf extract of cleistopholis patens (fam. Annonaceae) on candida species isolated from stage II HIV patients.|
Okechukwu, David C.; Momoh, Mumuni A. & Esimone, Charles O.
Background: Candida species (sp) is implicated in causing opportunistic disseminated mycotic complications in stage II
HIV patients. Cleistopholis patens is a West African medicinal tree reported to have significant antifungal activity against C.
Objectives: This study aimed to determine the anti-candidal activity of methanolic leaf extract of Cleistopholis patens
against Candida species isolated from stage II HIV patients.
Methods: The minimum inhibitory concentration (MIC) of the extract and Nystatin®® was determined by agar dilution
method. The killing rate studies of the plant extract and Nystatin® were also determined.
Results: The extract had activity against all Candida isolates, with the MIC against the five isolates ranging from 6.0 - 9.8
mg/ml. Nystatin® also demonstrated plausible activity against the isolates with MICs ranging from 0.3125 – 25 mg/ml.
Candida albicans strain 2 was the most sensitive to both extract and Nystatin® with MIC values of 6 and 0.3125 mg/ml
respectively. Candida krusei was the least sensitive with MIC values of 9.8 and 25 mg/ml for the extract and Nystatin®
respectively. The killing rate values for the extract ranged from -0.029 to -0.091 min-1 and that of Nystatin® ranged from
-0.076 to –0.11216 min-1.
Conclusions: The results indicate that the methanolic extract of Cleistopholis patens is a promising clinical alternative besides
Nystatin® in the treatment of infections caused by Candida species in stage II HIV patients.
Anti-candidal activity, Cleistopholis patens, Candida, HIV Patients