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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 15, No. 3, 2015, pp. 888-895
Bioline Code: hs15121
Full paper language: English
Document type: Study
Document available free of charge

African Health Sciences, Vol. 15, No. 3, 2015, pp. 888-895

 en Antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens
Adeniyi, Bolanle A.; Adetoye, Adewale & Ayeni, Funmilola A.

Abstract

Background: The addition of sub therapeutic doses of antibiotics to cattle feed for growth promotion is a contributory factor to antibiotic resistance, thus an alternative to antibiotics is needed in animal feed additives.
Objective: To determine the antimicrobial activity of cow’s intestinal Lactic acid bacteria (LAB) against enteric commensals.
Method: Escherichia coli check for this species in other resources , Klebsiella check for this species in other resources species (spp) and LAB were isolated from thirty different cow faecal samples and the LAB identified by partial sequencing of 16S rRNA. The antimicrobial activity of the LAB was determined against the test Escherichia coli and Klebsiella spp.
Results: Five species of LAB were isolated from thirty cow faecal samples and identified as Enterococcus hirae check for this species in other resources (8), Enterococcus durans check for this species in other resources (6), Enterococcus faecium check for this species in other resources (1), Enterococcus faecalis check for this species in other resources (1) and Weissella confusa check for this species in other resources (1). Viable cells and cell free supernatant (CFS) of the LAB were able to inhibit the growth of the test organisms with the largest zone of inhibition by the viable cells being 26mm against Escherichia coli CB6 produced by Enterococcus hirae CO6A while Weissella confusa CO29M and Enterococcus hirae CO2A produced the largest zones of inhibition (26mm) against Klebsiella CB2.
Conclusion: This study shows that LAB from cow faeces possess considerable antimicrobial activity against resistant Escherichia coli from the same environment.

Keywords
Antibacterial activities, lactic acid bacteria, cow faeces, enteric pathogens

 
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