The addition of sub therapeutic doses of antibiotics to cattle feed for growth promotion is a contributory
factor to antibiotic resistance, thus an alternative to antibiotics is needed in animal feed additives.
To determine the antimicrobial activity of cow’s intestinal Lactic acid bacteria (LAB) against enteric commensals.
Method: Escherichia coli
species (spp) and LAB were isolated from thirty different cow faecal samples and the
LAB identified by partial sequencing of 16S rRNA. The antimicrobial activity of the LAB was determined against the test
Five species of LAB were isolated from thirty cow faecal samples and identified as Enterococcus hirae
(8), Enterococcus durans
(6), Enterococcus faecium
(1), Enterococcus faecalis
(1) and Weissella confusa
(1). Viable cells and cell free
supernatant (CFS) of the LAB were able to inhibit the growth of the test organisms with the largest zone of inhibition by
the viable cells being 26mm against Escherichia coli
CB6 produced by Enterococcus hirae
CO6A while Weissella confusa
CO29M and Enterococcus hirae
CO2A produced the largest zones of inhibition (26mm) against Klebsiella
This study shows that LAB from cow faeces possess considerable antimicrobial activity against resistant Escherichia
from the same environment.