African Health Sciences
Makerere University Medical School
Vol. 15, No. 3, 2015, pp. 982-985
Bioline Code: hs15133
Full paper language: English
Document type: Research Article
Document available free of charge
African Health Sciences, Vol. 15, No. 3, 2015, pp. 982-985
© Copyright 2015 - African Health Sciences
Tryptophan-induced pathogenesis of breast cancer|
Cao, Zhu-Gen; Qin, Xiao-Bing; Liu, Fang-Fang & Zhou, Lei-Lei
Background: The pathogenesis of breast cancer remains unclear.
Aims: To investigate the pathogenesis of breast cancer through targeted metabolomics of amino acids components in serum
of patients with breast cancer.
Methods: Patients with breast cancers were enrolled in our hospital between year January 1st, 2013 and December 31st, 2014.
Targeted analysis of amino acids was performed using ESI-QTOF-MS instrument. In vitro experiment was performed to
determine the influence of tryptophan towards interleukin-10 (IL-10) secretion by CD4+ T cell.
Results: Targeted metabolomics of amino acids showed that the level of tryptophan significantly (p<0.05) increased in
patients with breast cancer. Furthermore, the biological function of tryptophan was determined through determining the
influence of tryptophan towards IL-10 secretion using in vitro method. The addition of tryptophan (100 μM) in the cell
medium can significantly inhibited the secretion of IL-10 by CD4+ T cells, as indicated by the mRNA level and protein
Conclusion: The inhibition of IL-10 secretion by CD4+ T cells is a potential pathogenesis of breast cancer.
breast cancer, interleukin-10 (IL-10), pathogenesis, T cell