Lime extracts of powdered combination of seeds of Picralima nitida
, stem bark of Alstonia boonei
leaves of Gongronema latifolium
is a common remedy used in the treatment of malaria in South Western Nigeria.
To determine the antiplasmodial activities of the combined herbal extracts and its impact on the haematological,
hepatological and renological parameters in mice.
The 4-day suppressive and curative tests were used to assess the antiplasmodial activities of the extract in mice
infected with chloroquine-sensitive Plasmodium berghei
at concentration of 200mg/kg, 400mg/kg and 800mg/kg body
weight. The haematological parameters including red blood cells, white blood cells, packed cell volume and haemoglobin
count were analysed with an auto analyser. The activities of alanine aminotransferase (ALT), aspartate aminotransferase
(AST) and alkaline phosphatase (ALP) were determined, while urea, protein and creatinine were analysed by standard procedural
The 4-day suppressive test revealed that the test extract achieved percentage suppression of 39.0%, 41.6% and
54.68% for the 200mg/kg, 400mg/kg and 800mg/kg concentration respectively. Additionally, the curative test achieved a
high percentage suppression of 80.97%, 83.84% and 86.16% at the 200mg/kg, 400mg/kg and 800mg/kg concentration respectively.
The extracts did not induce significant change on haematological parameters (P>0.05), while significant elevation
in the values of the ALT and AST (P<0.05) was observed and elevation of creatinine (P<0.05) at 800mg/kg.
The results support the traditional use of the herbal combination in the treatment of malaria, however the
liver cells were impacted by the extracts in bioassay conducted with mice.