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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 15, No. 4, 2015, pp. 1277-1281
Bioline Code: hs15175
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 15, No. 4, 2015, pp. 1277-1281

 en Occurrence of aminoglycoside-modifying enzymes genes (aac(6′)-I and ant(2′′)-I) in clinical isolates of Pseudomonas aeruginosa check for this species in other resources from Southwest Nigeria.
Odumosu, Bamidele Tolulope; Adeniyi, Bolanle A & Chandra, Ram


Background: Enzymatic modification of aminoglycosides is the primary mechanism of resistance by Pseudomonas aeruginosa check for this species in other resources . Obejectives: We investigated the occurrence and mechanism of aminoglycosides resistance in P. aeruginosa isolates from hospitals in SouthWest Nigeria.
Methods: A total of 54 consecutive, non-duplicate clinical isolates of P. aeruginosa were studied for the presence of aminoglycosides -modifying enzymes (AMEs) by PCR amplification and sequencing of genes encoding AMEs.
Results and Conclusion: Two types of AME genes [aac (6′) – I and ant (2′′) – I] were found in 12 isolates out of 54. Seven strains harboured one or more types of enzymes of which aac (6′) – I was the most frequently found gene (10/54 isolates, 18.5%). None of the isolates investigated in this study were positive for aph, aac (3) and aac (6′′) – II genes. Prevalence of P. aeruginosa producing AME genes in this study may suggest aminoglycosides use in Nigeria. This study highlights need for functional antimicrobial surveillance system in Nigeria.

Aminoglycoside-modifying enzymes; antibiotics resistance; Pseudomonas aeruginosa

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