About Bioline  All Journals  Testimonials  Membership  News

African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 15, No. 4, 2015, pp. 1282-1288
Bioline Code: hs15176
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 15, No. 4, 2015, pp. 1282-1288

 en Comparison of identification and antimicrobial resistance pattern of Staphylococcus aureus check for this species in other resources isolated from Amassoma, Bayelsa state, Nigeria.
Abidemi Ayeni, Funmilola; Gbarabon, Tombari; Andersen, Camilla & Nørskov-Lauritsen, Niels


Background: Staphylococcus aureus check for this species in other resources is often responsible for fatal infections and recent upsurge of resistant strains has resulted in therapeutic failure. The identification of this microorganism is a major challenge to medical microbiologists in developing countries.
Methods: One hundred and eighty five isolates which had been previously isolated from the nares of 185 healthy college students’ volunteers in Amassoma, Bayelsa State, South Nigeria were identified by MALDI TOF mass spectrometry, and PCR amplification of the spa gene. The identified isolates were compared with presumptive identities obtained by growth on MSA, tube coagulation and slide agglutination tests. Antimicrobial susceptibility testing of S. aureus isolates was performed by Kirby Bauer technique while MRSA was screened for by growth on chromIDTM MRSA plate and confirmed by PCR-amplification of mecA/mecC genes.
Results: From the 185 staphylococci that grew with yellow colonies on MSA, 24 were positive in the slide coagulase test, while 17 were positive in the tube coagulase test; MALDI TOF mass spectrometry and PCR amplification of the spa gene showed excellent concordance with the tube test, as all tube coagulase-positive strains were identified as S. aureus, while tube coagulase-test negative isolates in all cases were designated as other staphylococcal species by MALDI-TOF mass spectrometry and were spa PCR test negative. All S. aureus isolates were susceptible to clindamycin, vancomycin, fusidic acid, rifampicin and linezolid, while observed resistance to penicillin and trimethoprim were high. Only one MRSA strain was detected
Conclusion: The study confirms that the tube coagulase test is an accurate diagnostic method for identification of S. aureus, while growths on MSA and slide agglutination tests are inaccurate. We found a low prevalence of MRSA and a high rate of trimethroprim-resistance in the studied population.

Antibiotics; coagulase; identification; S. aureus

© Copyright 2015 - African Health Sciences

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2023, Site last up-dated on 01-Sep-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Google Cloud Platform, GCP, Brazil