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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 16, No. 2, 2016, pp. 629-639
Bioline Code: hs16079
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 16, No. 2, 2016, pp. 629-639

 en Antioxidant and antimicrobial activities of solvent fractions of Vernonia cinerea (L.) Less leaf extract.
Sonibare, Mubo A.; Aremu, Oluwafunmilola T. & Okorie, Patricia N.


Background: Vernonia cinerea (L.) Less is used in folk medicine as a remedy for various diseases.
Objectives: The present study reports antioxidant and antimicrobial activities of solvent fractions of Vernonia cinerea.
Methods: The antioxidant properties of solvent fractions of V. cinerea were evaluated by determining radicals scavenging activity, total flavonoid and phenolic contents measured with the 2,2-diphenyl-1-picryl hydrazyl (DPPH) test, the aluminum chloride and the Folin-ciocalteau methods, respectively. Antimicrobial activities were tested against human pathogenic microorganisms using agar diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each active extract were determined.
Results: The ethyl acetate fraction having the IC50 value of 6.50 μg/mL demonstrated comparable DPPH radical-scavenging activity with standard antioxidants, gallic acid and quercetin included in the study. All fractions displayed moderate antimicrobial potential against the tested pathogens with the zone of inhibition that ranged from 9.0 to 13.5 mm. The MIC (1.56 mg/mL) and MBC (3.13 mg/mL) indicated highest susceptibility of Candida albicans check for this species in other resources in all fractions.
Conclusion: The results of this study showed that the solvent fractions of V. cinerea possess antioxidant and antimicrobial activities, hence justifying the folkloric use of the plant for the treatment of various ailments in traditional medicine.

Vernonia cinerea; antioxidant activity; antimicrobial activity; total flavonoid content; total phenolic content

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