Population genomics diversity of Plasmodium falciparum in malaria patients attending Okelele Health Centre, Okelele, Ilorin, Kwara State, Nigeria.|
Kolawole,Olatunji Matthew; Mokuolu,Olugbenga Ayodeji; Olukosi,Yetunde Adeola & Oloyede,Tolulope Ololade
Background: Plasmodium falciparum, the most dangerous malaria parasite species to humans remains an important public health
concern in Okelele, a rural community in Ilorin, Kwara State, Nigeria. There is however little information about the genetic
diversity of Plasmodium falciparum in Nigeria.
Objective: To determine the population genomic diversity of Plasmodium falciparum in malaria patients attending Okelele Community
Healthcare Centre, Okelele, Ilorin, Kwara State.
Methods: In this study, 50 Plasmodium falciparum strains Merozoite Surface Protein 1, Merozoite Surface Protein 2 and Glutamate
Rich Protein were analysed from Okelele Health Centre, Okelele, Ilorin, Nigeria. Genetic diversity of P. falciparum isolates were
analysed from nested polymerase chain reactions (PCR) of the MSP-1 (K1, MAD 20 and RO33), MSP-2 (FC27 and 3D7) and
Glutamate Rich Protein allelic families respectively.
Results: Polyclonal infections were more in majority of the patients for MSP-1 allelic families while monoclonal infections were
more for MSP-2 allelic families. Multiplicity of infection for MSP-1, MSP-2 and GLURP were 1.7, 1.8 and 2.05 respectively
Conclusion: There is high genetic diversity in MSP – 2 and GLURP allelic families of Plasmodium falciparum isolates from Okelele
Health Centre, Ilorin, Nigeria.
Plasmodium falciparum; Merozoite Surface Protein; genetic diversity