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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 16, No. 3, 2016, pp. 734-743
Bioline Code: hs16093
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 16, No. 3, 2016, pp. 734-743

 en Anticancer and antibacterial secondary metabolites from the endophytic fungus Penicillium sp. check for this species in other resources CAM64 against multi-drug resistant Gram-negative bacteria.
Jouda,Jean-Bosco; Tamokou,Jean-de-Dieu; Mbazoa,Céline Djama; Sarkar,Prodipta; Bag,Prasanta Kumar & Wandji,Jean


Background: The emergence of multiple-drug resistance bacteria has become a major threat and thus calls for an urgent need to search for new effective and safe anti-bacterial agents.
Objectives: This study aims to evaluate the anticancer and antibacterial activities of secondary metabolites from Penicillium sp. check for this species in other resources , an endophytic fungus associated with leaves of Garcinia nobilis check for this species in other resources .
Methods: The culture filtrate from the fermentation of Penicillium sp. was extracted and analyzed by liquid chromatography– mass spectrometry, and the major metabolites were isolated and identified by spectroscopic analyses and by comparison with published data. The antibacterial activity of the compounds was assessed by broth microdilution method while the anticancer activity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
Results: The fractionation of the crude extract afforded penialidin A-C (1-3), citromycetin (4), p-hydroxyphenylglyoxalaldoxime (5) and brefelfin A (6). All of the compounds tested here showed antibacterial activity (MIC = 0.50 – 128 μg/mL) against Gramnegative multi-drug resistance bacteria, Vibrio cholerae check for this species in other resources (causative agent of dreadful disease cholera) and Shigella flexneri check for this species in other resources (causative agent of shigellosis), as well as the significant anticancer activity (LC50 = 0.88 – 9.21 μg/mL) against HeLa cells.
Conclusion: The results obtained indicate that compounds 1-6 showed good antibacterial and anticancer activities with no toxicity to human red blood cells and normal Vero cells.

Garcinia nobilis; endophytic fungi; secondary metabolites; antibacterial activity; anticancer activity

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