African Health Sciences
Makerere University Medical School
Vol. 17, No. 1, 2017, pp. 164-174
Bioline Code: hs17021
Full paper language: English
Document type: Research Article
Document available free of charge
African Health Sciences, Vol. 17, No. 1, 2017, pp. 164-174
© Copyright  - African Health Sciences
Renal histoarchitectural changes in nevirapine therapy: possible role of kolaviron and vitamin C in an experimental animal model.|
Offor, Ugochukwu; Ajayi, Sunday Adelaja; Jegede, Isaac Ayoola; Kharwa, Salem; Naidu, Edwin Coleridge & Azu, Onyemaechi Okpara
Background: There is paucity of literature regarding the nephrotoxicity of antiretroviral drugs and its interaction with plant-based
adjuvants. This study investigates the attenuating effect of kolaviron in nevirapine- therapy on the histological structure
of the kidneys of adult male Sprague-Dawley rats.
Objective: To determine the attenuating influence of anti-oxidant status of kolaviron on the kidneys of experimental animals
following nevirapine administration.
Methods: Forty eight pathogen-free adult male Sprague-Dawley rats were used for this study. The animals were divided into
8 groups (A-H) with 6 animals in each group. Group A was given normal saline as the control; group B was given nevirapine;
group C was given kolaviron; group D was given vitamin C; group E was given nevirapine and kolaviron; group F was given nevirapine
and vitamin C; Group G was given nevirapine and kolaviron (kolaviron withdrawn after day 28) and group H was given
corn oil. The experiment lasted 56 days after which the animals were sacrificed, blood samples were collected through cardiac
puncture for serum analysis and the kidneys were harvested and prepared for H& E histological examination.
Results: Nevirapine caused histoarchitectural damage in the glomerular apparatus with resultant increase in kidney/body
weight ratio (p<0.001). Adjuvant treatment with kolaviron attenuated these nephrotoxic effects. Serum anti-oxidant enzyme
(SOD and CAT) activities were significantly reduced in kolaviron and vitamin C treated animals, whereas in the nevirapine group
these parameters were significantly elevated (P<0.05). However, co-administration of nevirapine and vitamin C did not improve
the histoarchitecture of the kidney.
Conclusion: Adjuvant treatment with kolaviron (an anti-oxidant) for 56 days appears to attenuate the nephrotoxicity of nevirapine
in this model.
Kidney; histoarchitecture; kolaviron; antiretroviral drugs