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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 17, No. 1, 2017, pp. 199-207
Bioline Code: hs17025
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 17, No. 1, 2017, pp. 199-207

 en Quorum sensing inhibitory activity of sub-inhibitory concentrations of β-lactams
El-Mowafy, Somaia A.; Abd El Galil, Khaled H.; Habib, El-Sayed E. & Shaaban, Mona I.


Introduction: The virulence factors of Pseudomonas aeruginosa check for this species in other resources are under the control of quorum sensing (QS) signals. Hence, interference with QS prevents its pathogenesis.
Objective: The aim of the present research is to assess the influence of some β-lactam antibiotics on cell communication and the release of different virulence factors.
Methods: The minimal inhibitory concentrations of ceftazidime, cefepime and imipenem were evaluated by microbroth dilution method. The effect of sub-inhibitory concentration of the tested antibiotics on QS signals was investigated using reporter strain assay. In addition, different virulence factors (elastase, protease, pyocyanin and hemolysin) were estimated in the presence of their sub-inhibitory concentrations.
Results: Low concentrations of ceftazidime, cefepime and imipenem caused significant elimination of the QS signals 3OHC12-HSL and C4-HSL up to 1/20 MIC. Furthermore, low concentrations of the tested antimicrobials suppressed virulence factors elastase and hemolysin. Moreover, 1/20 of their MICs reduced elastase, protease, pyocyanin and hemolysin.
Conclusion: Utilization of β-lactam antibiotics at low concentrations could be an effective approach for prevention and treatment of P. aeruginosa infection.

Quorum sensing inhibition; β-lactams; Pseudomonas aeruginosa

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