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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 17, No. 3, 2017, pp. 808-819
Bioline Code: hs17100
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 17, No. 3, 2017, pp. 808-819

 en Repurposing metformin as a quorum sensing inhibitor in Pseudomonas aeruginosa check for this species in other resources
Abbas, Hisham A; Elsherbini, Ahmed M & Shaldam, Moutaz A


Background: Quorum sensing is a mechanism of intercellular communication that controls the production of virulence factors in Pseudomonas aeruginosa. Inhibition of quorum sensing can disarm the virulence factors without exerting stress on bacterial growth that leads to emergence of antibiotic resistance.
Objectives: Finding a new quorum sensing inhibitor and determining its inhibitory activities against virulence factors of Pseudomonas aeruginosa PAO1 strain.
Methods: Quorum sensing was evaluated by estimation of violacein production by Chromobacterium violaceum check for this species in other resources CV026. Molecular docking was used to investigate the possible binding of metformin to LasR and rhlR receptors. The inhibition of pyocyanin, hemolysin, protease, elastase in addition to swimming and twitching motilities, biofilm formation and resistance to oxidative stress by metformin was also assessed.
Results: Metformin significantly reduced the production of violacein pigment. Significant inhibition of pyocyanin, hemolysin, protease and elastase was achieved. Metformin markedly decreased biofilm formation, swimming and twitching motilities and increased the sensitivity to oxidative stress. In the molecular docking study, metformin could bind to LasR by hydrogen bonding and electrostatic interaction and to rhlR by hydrogen bonding only.
Conclusion: Metformin can act as a quorum sensing inhibitor and virulence inhibiting agent that may be useful in the treatment of Pseudomonas aeruginosa infection.

Metformin; Pseudomonas aeruginosa; quorum sensing; virulence inhibition.

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