African Health Sciences
Makerere University Medical School
Vol. 18, No. 2, 2018, pp. 304-312
Bioline Code: hs18040
Full paper language: English
Document type: Study
Document available free of charge
African Health Sciences, Vol. 18, No. 2, 2018, pp. 304-312
© Copyright 2018 - Rampersad et al.
Repeatability and reproducibility of retinal nerve fibre layer thickness measurements with the iVue-100 optical coherence tomographer|
Rampersad, Nishanee & Hansraj, Rekha
Background: Accurate and repeatable measurements of the retinal nerve fibre layer (RNFL) thickness are important in the
diagnosis and management of glaucoma and other disorders.
Objective: To assess the repeatability and reproducibility of the iVue-100 optical coherence tomographer (OCT).
Methods: The thickness of the RNFL was measured for 50 healthy participants using the iVue-100 OCT. Although both eyes
per participant were measured, only right eyes were analysed here. Repeatability and reproducibility of the iVue-100 OCT were
assessed using the intraclass correlation coefficient (ICC), coefficient of variation (CoV), paired t-tests and Bland-Altman analysis.
Results: Good intra-observer repeatability was obtained as indicated by the ICC of observer 1 (range: 0.941 - 0.976) and observer 2 (range: 0.829 – 0.953) as well by the CoV of observer 1 (range: 0.098 – 0.137) and observer 2 (0.091 – 0.132). In terms
of inter-observer reproducibility, significant differences (p< 0.05) in mean measurements between the observers were noted for
the average RNFL readings and in the superior and inferior quadrants as assessed with paired t-tests. Even though significant
inter-session differences were found for the average RNFL thickness and the superior quadrant (p = 0.003 and p = 0.013, respectively), excellent ICCs were obtained for inter-session reproducibility (range: 0.914 – 0.979).
Conclusion: The iVue-100 OCT demonstrated good repeatability and reproducibility for RNFL thickness measurements.
Retinal nerve fibre layer thickness; optical coherence tomography; repeatability; reproducibility; iVue-100 OCT