African Health Sciences
Makerere University Medical School
Vol. 18, No. 3, 2018, pp. 653-663
Bioline Code: hs18082
Full paper language: English
Document type: Study
Document available free of charge
African Health Sciences, Vol. 18, No. 3, 2018, pp. 653-663
© Copyright 2018 - Obembe et al.
Effects of aqueous extract of Moringa oleifera seed on cadmium-induced reproductive toxicity in male Wistar rats|
Obembe, Olawale O & Raji, Yunus
Bacground: Moringa oleifera seeds have been reported to increase sexual activity of male rats but its mechanism of action remains
Objective: Cadmium is a heavy metal that induces reproductive toxicity. To elucidate its possible mechanisms of action, the
effects of aqueous extract of Moringa seeds (AEMS) on Cadmium-induced reproductive damage were investigated.
Methods: Thirty male rats (180-200g, n=5) were grouped as follows: Control, Cadmium (2mg/kg), AEMS (100 and 500mg/
kg), Cadmium+100mg/kg AEMS and Cadmium+500mg/kg AEMS. Single intraperitoneal dose of Cadmium was administered
while AEMS was given daily (p.o) for 6 weeks and thereafter sacrificed by cervical dislocation. Sperm variables were examined
microscopically while serum was analysed for sex hormones and antioxidants, testicular and epididymal total protein from tissue
homogenate. Data were analysed using Students t-test and ANOVA.
Results: Cadmium caused significant decrease in sperm variables. The AEMS decreased sperm motility, count and testosterone.
These declines were dose dependent. Total testicular protein was significantly lowered by AEMS but total epididymal protein
was not affected. Serum catalase and SOD was reduced by AEMS while MDA increased.
Conclusion: Moringa seeds may exhibit male reproductive toxicity, as observed from its deleterious effect on androgen and
sperm variables. It failed to prevent cadmium-induced alteration in reproductive variables and serum antioxidants.
Aqueous extract; Moringa oleifera seed; cadmium-induced reproductive toxicity; male Wistar rats.