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Resistance status to deltamethrin pyrethroid of Culex pipiens pipiens (Diptera: Culicidae) collected from three districts of Tunisia Tabbabi, Ahmed; Daaboub, Jabeur; Cheikh, Raja Ben; Laamari, Ali; Feriani, Mohamed; Boubaker, Chokri; Jha, Ibtissem Ben & Cheikh, Hassen Ben Abstract Objectives: The aim of the present study was to determine the susceptibility status of Culex pipiens pipiens populations against deltamehtrin insecticide. Methods: Larvae of Culex pipiens pipiens were collected from three breeding places in Northern and Southern Tunisia between 2003 and 2005. Early third and late fourth instars were tested against deltamethrin pyrethroid insecticide. Cross-resistance with DDT resistance was evaluated in studied samples to estimate the role of target site insensitivity and two synergists including piperonyl butoxide (Pb) and S,S,S-tributyl phosphorotrithioate (DEF) were used to estimate the role of detoxification enzymes. Results: Our results revealed that the level of deltamehtrin resistance ranged from 0.67 to 31.4. We also showed the non-involvement of kdr resistance in pyrethroid resistance and no cross-resistance with DDT resistance was detected in all studied populations including the most resistant one. Synergists study on the resistant population (sample # 1) showed the involvement of CYP450 in the recorded resistance to the deltamethrin insecticide. Conclusion: The results obtained from this study should be considered in the current control programs to combat mosquitoes in Tunisia. Keywords Culex pipiens pipiens; deltamethrin resistance; kdr mutation; detoxification; Tunisia

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