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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 19, No. 1, 2019, pp. 1346-1352
Bioline Code: hs19005
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 19, No. 1, 2019, pp. 1346-1352

 en Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker
Shahnazi, Mojtaba; Mehrizi, Majid Zarezadeh; Alizadeh, Safar Ali; Heydarian, Peyman; Saraei, Mehrzad; Alipour, Mahmood & Hajialilo, Elham


Introduction: Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method.
Methods: A total of 1521 stool samples were collected from the study individuals. Initially, the identification of hymenolepis nana was confirmed by parasitological method including direct wet-mount and formalin-ethyl acetate concentration methods. Afterward, PCR-sequencing analysis of ribosomal ITS2 fragment was targeted to investigate the molecular identification of the parasite.
Results: Overall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration. All ten isolates were succefully amplified by PCR and further sequenced. The determined sequences were deposited in GenBank under the accession numbers MH337810 -MH337819.
Conclusion: Our results clarified the presence of H. nana among the patients in the study areas. In addition, the molecular technique could be accessible when the human eggs are the only sources available to identify and diagnose the parasite.

Cite as: Shahnazi M, Mehrizi MZ, Alizadeh SA, Heydarian P, Saraei M, Alipour M, Hajialilo E. Molecular characterization of Hymenolepis nana (Cestoda: Cyclophyllidea: Hymenolepididae) based on nuclear rDNA ITS2 gene marker. Afri Health Sci. 2019;19(1): 1346- 1352.

Hymenolepis nana; rDNAITS; PCR; Iran

© Copyright 2019 - Shahnazi et al.

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