are ubiquitous bacteria causing many clinical conditions including acute diarrhea. Diarrheagenic Aeromonas harbors aerolysin gene secreting virulent enterotoxin, aerolysin.
To develop a molecular and immunological based method for detection of Aeromonas
strains were identified from stool samples using culture, enterotoxicity testing using mice model.
During immune magnetic polymerase chain reaction IM-PCR protocol, aerolysin specific antibodies were bound with immuno
magnetic binding. Sensitivity and specificity tests for IM-PCR were conducted.
There was high detection of Aeromonas
using IM-PCR (12.4 %) technique when compared to low isolation with culture (5.1%). Our study confirmed that some strains of enterotoxic Aeromonas
strains were uncultivable. Enterotoxicity tests on
culture isolates revealed many strains were negative. IM-PCR detected high, (62/500) rate of identification of Aeromonas
aerolysin toxin gene. Aeromonas
species identified after IM-PCR were
(9.6 %) and
(6.4%). All A. trota
strains were undetected by cultivation.
High sensitivity and specificity of IM-PCR are due to preparation of aerolysin antibodies and immuno magnetic
binding, prior to PCR. Since diseases due to Aeromonas
are increasingly reported, IM-PCR is recommended for detection from
Cite as: Subbaram K, Gatasheh MK, Al Azzam KM, Kannan H. Molecular identification of diarrheal Aeromonas using immuno magnetic
polymerase chain reaction (IM-PCR) technique: a comparative study with conventional culture method. Afri Health Sci.2019;19(2): 2036-2042.