Quorum sensing inhibitionis an advanced strategy that aims to interfere with bacterial cell-to-cell communication systems (quorum sensing), which regulate virulence factors production in Pseudomonas aeruginosa
, in order to overcome the globalcrisis of antimicrobial resistance.
Study the potential quorum sensing inhibitory effect of Zinc oxide (ZnO)nanoparticlesin Pseudomonas aeruginosa
and the impact on production of virulence factors.
Quorum sensing inhibitory effect of ZnO was evaluated by assessing its ability to reducePseudomonas aeruginosa
virulence factors production; rhamnolipids, pyocyanin, pyoverdin, hemolysins, elastase and proteases. Furthermore, qRT-PCR
was performed to determine ZnO inhibitory effect onQS-regulatory geneslasI, lasR,rhlI, rhlR, pqsA and pqsR that control
virulence factors secretion. Moreover, mice survival test was conducted to investigate the influence of ZnO on Pseudomonas aeruginosa-induced mortality in vivo.
ZnO revealed a statistically significant reduction in the production of QS-controlled virulence factors rhamnolipids,
pyocyanin, pyoverdin, hemolysins, elastase and proteases. Furthermore, ZnO exhibited a significant decrease in the relative expression of QS-regulatory geneslasI, lasR,rhlI, rhlR,pqsA and pqsR. Additionally, ZnO significantly reduced the pathogenesis
of Pseudomonas aeruginosa
ZnO nanoparticles can be used as a quorum sensing inhibitor in Pseudomonas aeruginosa
infections either as an adjuvant or alternative to conventional antimicrobials.
Cite as: Saleh MM, Sadeq RA, Abdel Latif HK, Abbas HA, Askoura M. Zinc oxide nanoparticles inhibits quorum sensing and virulence in
Pseudomonas aeruginosa. Afri Health Sci.2019;19(2): 2043-2055. https://dx.doi.org/10.4314/ahs.v19i2.28