African Health Sciences
Makerere University Medical School
Vol. 19, No. 4, 2019, pp. 2846-2853
Bioline Code: hs19152
Full paper language: English
Document type: Study
Document available free of charge
African Health Sciences, Vol. 19, No. 4, 2019, pp. 2846-2853
© Copyright 2019 - Belgacem et al.
Exploration of hypoglycemic effect of an extract from leaves of a plant from Tunisian pharmacopeia: Artemisia campestris (Asteraceae)|
Belgacem, Amel; Gdara, Neyla Ben; Khemiri, Ikram & Bitri, Lotfi
Background and objectives: A lot of research has been directed towards medicinal plants which are considered as a source
of multiple phytotherapic substances endowed with hypoglycemic activities that could be used to treat diabetes and its complications.
Our study was carried out in Wistar rats to investigate the hypoglycemic effect of n-Butanol Fraction from Artemisia
campestris leaf Methanolic Extract (BFACME).
Methods: Two experimental models were used in rats: orally induced hyperglycemia (OGTT) and isolated perfused liver (IPRL).
Results: BFACME at 550 mg/kg BW dose significantly reduced fasting glucose level in normal rats as compared to controls.
The decrease of glycaemia was 12.6% more significant than that obtained with the standard drug glibenclamide (10 mg/kg BW),
an oral antidiabetic preparation belonging to sulfonylurea class. In OGTT model, BFACME at the highest doses of 550 and
400 mg/kg BW significantly reduced the postprandial hyperglycemic peak compared to controls. In the IPRL model, treatment
with BFACME significantly decreased glucose concentrations after 30 min of perfusion with 30 mM glucose solely when insulin
was present. The higher doses of BFACME lead to glucose concentration at basal level as early as 90 min, while the lowest dose
does not restore this concentration even to t = 120min. The best initial glucose concentration retrieval was obtained with 0.7
mg BFACME/mL/g liver. At this dose, BFACME improves the decrease of glucose level caused by only insulin by about 18%.
Conclusion: The BFACME appears to exert a hypoglycemic activity by potentiating the insulin action.
Artemisia campestris; hypoglycemic activity; isolated perfused rat liver; rats.