African Health Sciences
Makerere University Medical School
Vol. 19, No. 4, 2019, pp. 3242-3248
Bioline Code: hs19194
Full paper language: English
Document type: Research Article
Document available free of charge
African Health Sciences, Vol. 19, No. 4, 2019, pp. 3242-3248
© Copyright 2019 - Ndede et al.
Immunoglobulin heavy variable (IgHV) gene mutation and micro-RNA expression in Burkitt’s lymphoma at Moi Teaching and Referral Hospital in Western Kenya|
Ndede, Isaac; Mining, SK; Patel, K; Wanjala, FM & Tenge, C.
Introduction: Burkitt’s lymphoma (BL) is a virus associated childhood B-cell cancer common in Eastern Africa. Continued
survival of B-cells in germinal centres depend on expression of high affinity immunoglobulins (Ig) to complementary antigens
by somatic hypermutation of Ig genes. Cellular microRNAs, non-coding RNAs have been reported to play role in cell cycle
regulation. Both viral antigen dependent mutation and micro-RNA expression maybe involved in BL pathogenesis.
Objective: To describe immunoglobulin heavy variable (IgHV) rearrangement and micro-RNA expressions in BL tumours.
Methods: Genomic DNA were extracted and purified from BL tissue blocks at Moi Teaching and Referral Hospital, before
amplification using IgHV consensus primers and sequencing. The sequences were then aligned with germline alleles in IMGT/
V-QUEST® database. Total RNA extracted from tissue blocks and cell lines were used to determine relative expression of hsamiR-34a and hsa-miR-127.
Results: In all tumours, allele alignment scores and number of mutations range were 89.2-93.2%, 15-24 respectively. The range
of IgHV amino acid changes were higher in EBER-1+ (15-25) than EBER-1- (9-15). In MYC+ tumours, the relative expression
were: hsa-miR-127(2.09);hsa-miR-34a (2.8) and MYC- hsa-miR-127 (1.2), hsa-miR-34a (1.0).
Conclusion: B-cell in BL contained somatic mutated IgHV gene and upregulated cellular microRNAs with possible pathogenetic
IgHV; somatic hypermutation; microRNA; Burkitt’s lymphoma.