Background: In this study, it was aimed to investigate the effects of bacterial cells and cell-free filtrates of
Lactobacillus acidophilus
8MR7 and
Lactobacillus paracasei subspecies paracasei
10MR8 on the biofilm formation of 3
Candida tropicalis
, 3
C. glabrata
and 12
C. albicans
isolated from the vagina and identified their virulence factors.
Methods: Haemolytic activities esterase activities, and phospholipase activities as virulence factors of
Candida strains were determined. Biofilm formations of these isolates were determined by Congo Red agar and microtitration plate method. Antibiofilm activities of bacterial cells and cell-free filtrates of
L. acidophilus 8MR7 and
L. paracasei subspecies paracasei 10MR8 on Candida isolates were determined by the microtitration plate method.
Results: Bacterial cells of
L. acidophilus 8MR7 and
L. paracasei subspecies paracasei 10MR8 were not very effective in the inhibition of biofilm, whereas it has been observed that the cell-free filtrates of these bacteria inhibit the formation of biofilms of Candida strains. Although the main mechanism for inhibiting the formation of
Candida spp. biofilm is the competition for adhesion, it is concluded that the substances contained in the cell-free filtrates of lactic acid bacteria are also important.
Conclusion: These isolates promise hope as potential bacteria that can be used for anti-adhesion purposes in health-care materials.
