African Health Sciences
Makerere University Medical School
Vol. 20, No. 3, 2020, pp. 1090-1108
Bioline Code: hs20083
Full paper language: English
Document type: Review Article
Document available free of charge
African Health Sciences, Vol. 20, No. 3, 2020, pp. 1090-1108
© Copyright 2020 - Aruhomukama D.
Review of phenotypic assays for detection of extended-spectrum β-lactamases and carbapenemases: a microbiology laboratory bench guide|
Background: Infections caused by gram-negative antibiotic-resistant bacteria continue to increase. Despite recommendations
by the Clinical Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility
Testing (EUCAST) with regards to detection of antibiotic degrading enzymes secreted by these bacteria, the true prevalence
of extended-spectrum β-lactamase (ESBL) and carbapenemase producers remains a difficult task to resolve. Describing of
previously designed phenotypic detection assays for ESBLs and carbapenemases in a single document avails a summary that
allows for multiple testing which increases the sensitivity and specificity of detection.
Methods and aims: This review, therefore, defined and classified ESBLs and carbapenemases, and also briefly described
how the several previously designed phenotypic detection assays for the same should be performed.
Conclusion: Extended-spectrum β-lactamase and carbapenemase detection assays, once performed correctly, can precisely
discriminate between bacteria producing these enzymes and those with other mechanisms of resistance to β-lactam antibiotics.
Extended-spectrum β-lactamases; carbapenemases; phenotypic detection.