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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 20, No. 3, 2020, pp. 1124
Bioline Code: hs20086
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 20, No. 3, 2020, pp. 1124

 en Epidemiological study of bacterial meningitis in Tunisian children, beyond neonatal age, using molecular methods: 2014-2017
Haddad-Boubaker, Sondes; Lakhal, Marwa; Fathallah, Cyrine; Mhimdi, Samar; Bouafsoun, Aida; Kechrid, Amel & Smaoui, Hanen


Background: Since the 1990s, the epidemiology of bacterial meningitis worldwide has changed thanks to vaccination. In Tunisia, the main causative pathogens were Streptococcus pneumoniae check for this species in other resources , Neisseria meningitidis check for this species in other resources and Haemophilus influenzae check for this species in other resources serotype b (Hib). Only Hib vaccination was available during our study period.
Objectives: We performed a laboratory case report based-study of suspected bacterial meningitis in Northern Tunisia from January 2014 to June 2017.
Methods: CSF samples obtained from children beyond neonatal age with suspicion of meningitis were tested by two real time PCRs, targeting pneumococcus, meningococcus and Hib, and conventional methods.
Results: Using real-time PCR, 63 were positive including ten supplementary cases compared to conventional methods. A general decrease of bacterial meningitis cases was demonstrated comparing to previous data. Pneumococcus was predominant (69.84%) followed by meningococcus (28.57%) and Hib (1.59%). The main serotypes were 14, 19F, 6B and 23F for pneumococcus and serogroup B for meningococcus. Most cases occurred during cold season and children under one year were the most affected by bacterial meningitis.
Conclusion: Our study suggests the predominance of pneumococcal cases. It may provide valuable data on meningitis epidemiology before the introduction of pneumococcal vaccine, which may be useful for future evaluation.

Bacterial meningitis; children; Tunisia; PCR.

© Copyright 2020 - Haddad-Boubaker S et al.

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