Detection, quantification and genotype distribution of HCV patients in Lahore, Pakistan by real-time PCR|
Khan, Muhammad Umer; Sadia, Haleema; Irshad, Asma; Baig, Atif Amin; Ashiq, Sana; Zahid, Beenish; Sheikh, Rozeena; Roshan, Sadia; Ali, Azam; Shamas, Shazia; Bhinder, Munir Ahmed & Ahmad, Rais
Background: Hepatitis C virus (HCV) is considered as “Viral Time Bomb” suggested by the World Health Organization
and if it is not treated timely, it will lead towards cirrhosis and hepatocellular carcinoma (HCC).
Objective: The purpose of the present research is to study possible risk factors, frequent genotypes of HCV and its association
with different age groups.
Methods: Suspected blood samples from HCV patients were collected from different hospitals of Lahore, Pakistan. Out
of 1000 HCV suspected samples, 920 samples were found HCV positive detected by Anti-HCV ELISA, CobasR. kit. The
quantification of HCV load was determined by HCV quantification kit and LINEAR ARRAY KIT (Roche) was used for
genotype determination by Real-Time PCR (ABI). Statistical analysis was done by using Microsoft Excel.
Results: Out of 920 subjects, 77 subjects (8.4%) were false positive and they were not detected by nested PCR. Three PCR
positive samples were untypeable. Genotype 3 was predominant in Lahore which was 83.5%, whereas type 1 and 2 were
5.1% and 0.7% respectively. There were also mixed genotypes detected, 1 and 3 were 0.4%, 2 and 3 were 1.41% and 3 and 4
were 0.2% only. Male were more infected of HCV in the age <40 years and females >40years.
Conclusion: The major risk factor for HCV transmission is by use of unsterilized razors/blades. It is necessary to spread
awareness among the general population of Pakistan about HCV transmission risk factors. Regular physical examination at
least once a year is recommended, so that early detection of HCV could be done.
Hepatitis C virus; hepatocellular carcinoma; quantification; genotype; real-time PCR.