Molecular detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary samples in a hospital-based study|
Goud, Kalal Iravathy; Kavitha, Matam; Mahalakshmi, Adi; Vempati, Ravi; Alodhayani, Abdulaziz A; Mohammed, Arif A & Khan, Imran Ali
Objective: Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a deadly infectious disease. India
contributes to one-third of the global TB burden. However, no studies have been carried out in the Telangana (Hyderabad)
population using real-time polymerase chain reaction (RT-PCR). Therefore, the current study evaluated the role of RT-PCR
as a rapid and non-invasive test to diagnose TB by testing for pulmonary tuberculosis (PTB) and extrapulmonary tuberculosis
Materials and methods: This hospital-based study examined 1670 samples (900 EPTB; 770 PTB) comprising tissue (n =
537), peritoneal fluid (n = 420), sputum (n = 166), bronchial fluid (n = 126), cerebrospinal fluid (n = 145), ascetic fluid (n
= 76), sputum pus (n = 78), urine (n = 79), and bronchoalveolar fluid (n = 43) samples. DNA from samples was separated
using specific isolation kits and subjected to RT-PCR.
Results: In this study, we enrolled 1670 subjects and categorized 54.4% as females and 45.6% as males. The collected samples
were categorized as 48.5% of fluid samples, followed by tissue (32.2%), sputum (9.9%), urine (4.7%), and pus-swab
(4.6%). RT-PCR analysis revealed that 4.7% patients were positive for Mtb. Our results revealed that 61% of the affected
patients were male and 39% were female. Among the specimen types, tissue samples gave the highest proportion of positive
Conclusion: The results showed that RT-PCR should be implemented and given top priority in TB diagnosis to save time
and facilitate a definitive diagnosis. Tissue samples are highly recommended to screen the Mtb through the technique RT-PCR.
Future studies should extend the technique to the global population and exome sequencing analysis should be performed
to identify TB risk markers.
Tuberculosis (TB); EPTB; PTB; Mycobacterium tuberculosis (Mtb).