Association of FADS2 rs174575 gene polymorphism and insulin resistance in type 2 diabetes mellitus|
Shetty, Shilpa S; Kumari, Suchetha N; Devi, Harshini; KP, Sharmila & Rai, Srinidhi
Background: Many risk factors contribute to the pathogenesis of diabetes. Gene and lifestyle factors are considered to be
the major contributors. A dietary pattern is attributed to be one of the lifestyle risk factors favoring diabetes. The present
study aims to find an association between fatty acid desaturase (FADS) gene polymorphism and glycemic profile in type 2
diabetes mellitus (T2DM).
Methodology: A total of 429 subjects were included in the study on the basis of inclusion and exclusion criteria, of which
213 and 216 subjects were diabetic and control, respectively. Body mass index was calculated. Fasting plasma glucose, glycated
hemoglobin (HbA1c) and insulin were measured using commercially available kits. rs174575 of FADS2 was selected
based on previous publications and identified using the dbSNP database. To compare the biochemical parameters with the
genotype, the following three models were used: additive model (CC vs CG vs GG), dominant model (CC + CG vs GG),
and recessive model (CC vs CG + GG).
Results and Discussion: FBS, HbA1c, insulin, HOMA-IR, and HOMA-B exhibited a high and statistically significant
difference between subjects and controls. The three models exhibited a statistically significant difference between FBS, HOMA-IR, and HOMA- B (p<0.05).
Conclusion: The distribution of rs174575 genotype differed significantly between the subjects and controls in the present
study. The study revealed that genetic variation in FADS2 is an additional facet to consider while studying the risk factors
Fatty acid desaturase; gene polymorphism; type 2 diabetes mellitus.