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African Health Sciences
Makerere University Medical School
ISSN: 1680-6905
EISSN: 1680-6905
Vol. 21, No. 1, 2021, pp. 8-14
Bioline Code: hs21007
Full paper language: English
Document type: Research Article
Document available free of charge

African Health Sciences, Vol. 21, No. 1, 2021, pp. 8-14

 en Association analysis of vitamin D receptor gene polymorphisms in North England population with Type 2 diabetes mellitus
Sattar, Naila Abdul; Shaheen, Sumera; Hussain, Fatma & Jamil, Amer

Abstract

Background: Numerous diabetes susceptibility loci, include a region consisting vitamin D receptor gene found in chromosome 12q, have been known using genome wide screens.
Aim: The aim of present study is to probe the relationship between polymorphism of vitamin D receptor gene (single nucleotide polymorphisms) and type 2 diabetes mellitus (T2DM). Five hundred T2DM patients and 200 healthy subjects with normal HbA1c( ≤ 5.0 %), fasting blood sugar (≤ 120 mg/dL) and random blood sugar (≤ 140 mg/dL) were enrolled.
Metholodgy: The genotypes were found by polymerase chain reaction restriction fragment length polymorphism and DNA sequencing.
Results: revealed that no considerable differences in frequencies of genotype and allele of the Bsm I and Fok I polymorphisms between healthy and patients in the North England (For Fok I: OR = 1.11, 95% CI: 0.72–1.12; for Bsm I: OR = 1.35, 95% CI: 0.79–1.98).
Conclusion: It is recommended that both following polymorphisms of vitamin D receptor gene may not considerably add to the progression of T2DM in the North England.

Keywords
T2DM; DNA Sequencing; Polymerase Chain Reaction-Restriction; Fragment Length Polymorphism (PCR-RFLP); Single nucleotide Polymorphism (SNP); VDR.

 
© Copyright 2021 - Sattar NA et al.

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