African Health Sciences
Makerere University Medical School
Vol. 21, No. 1, 2021, pp. 15-22
Bioline Code: hs21008
Full paper language: English
Document type: Research Article
Document available free of charge
African Health Sciences, Vol. 21, No. 1, 2021, pp. 15-22
© Copyright 2021 - Hameed T et al.
Associations of transcription factor 7-Like 2 (TCF7L2) gene polymorphism in patients of type 2 diabetes mellitus from Khyber Pakhtunkhwa population of Pakistan|
Hameed, Taha; Khan, Zahid; Imran, Muhammad; Ali, Saif; Albegali, Abdullah Abdo; Ullah, Muhammad Ikram & Ejaz, Hasan
Background: Type 2 diabetes mellitus (T2DM) is the most prevalent component of metabolic syndrome. Environmental
factors and various complex genes like transcription factor 7-like 2 (TCF7L2) gene have involved in the disease development.
Objective: To determine TCF7L2 genetic association (rs7903146C/T and rs12255372G/T) in T2DM patients of Khyber
Pakhtunkhwa population of Pakistan.
Subjects and methods: This study comprised of 176 subjects including 118 T2DM patients and 58 healthy controls.
Genomic DNA was extracted and genotype of common variants (rs7903146 C/T and rs12255372 G/T) was carried out by
amplification-refractory mutation system (ARMS)-PCR of sequence specific oligonucleotides.
Results: The distribution of genotype of TCF7L2 SNPs (rs7903146 C/T and rs12255372 G/T) was significantly associated
with T2DM as compared to the controls (p <0.0001). The genetic models of the rs7903146 (C/T) and rs12255372 (G/T)
SNPs were significantly associated between cases and controls (p <0.0001). On the other hand, the significant association
was observed between the two SNPs and different biochemical parameters like serum fasting glucose, lipid profile, creatinine
and blood HbA1c levels (p <0.05).
Conclusion: It is concluded that the SNPs of the TCF7L2 gene are significantly associated with T2DM disease susceptibility
in the population of Khyber Pakhtunkhwa of Pakistan.
T2DM; TCF7L2; Genetic association; ARMS-PCR; Single nucleotide Polymorphism (SNPs); Khyber Pakhtunkhwa.