African Health Sciences
Makerere University Medical School
Vol. 21, No. 2, 2021, pp. 505-512
Bioline Code: hs21044
Full paper language: English
Document type: Research Article
Document available free of charge
African Health Sciences, Vol. 21, No. 2, 2021, pp. 505-512
© Copyright 2021 - Mofolorunsho KC et al.
Prevalence and antimicrobial susceptibility of extended-spectrum beta lactamases-producing Escherichia coli and Klebsiella pneumoniae isolated in selected hospitals of Anyigba, Nigeria|
Mofolorunsho, Kehinde C; Ocheni, Hannah O; Aminu, Ruth F; Omatola, Cornelius A & Olowonibi, Olabisi O
Background: Escherichia coli and Klebsiella pneumoniae are commonly implicated in urinary tract infections accounting for
majority of the antimicrobial resistance encountered in hospitals.
Objectives: To determine the prevalence and antimicrobial susceptibility of extended-spectrum beta-lactamases (ESBLs)
producing E. coli and K. pneumoniae among patients in Anyigba, Nigeria.
Methods: This hospital-based cross-sectional study was conducted using urine samples from 200 patients of Grimmard
Catholic hospital and Maria Goretti hospital. Urine samples were processed to identify ESBL-producing E. coli and K. pneumoniae
using standard microbiological techniques. Isolates were then tested against antimicrobial agents.
Results: A total of 156 bacterial isolates were recovered consisting 128 of E. coli and 28 of K. pneumoniae. Extended spectrum
beta-lactamases production was observed in 69% of E. coli and 31% of K. pneumoniae. These pathogens were resistant
to 3 or more antibiotics. Of the antimicrobials tested, cefotaxime demonstrated the highest rates of resistance (100%) for
both ESBL-producing E. coli and K. pneumoniae. Fifty-four isolates of ESBL-producing E. coli showed a high level of resistance
to amoxicillin clavulanic acid (83.3%), ciprofloxacin (83.3%), and ceftazidime (79.6%). ESBL-positive K. pneumoniae isolates
were highly resistant to ciprofloxacin (75%), and amoxicillin clavulanic acid (83.3%). Cefoxitin (62.5%) and gentamicin
(66.7%) showed substantially higher rates of resistance against these isolates while all 24 strains were resistant to imipenem.
Conclusion: This study indicated the prevalence of ESBL-positive Gram-negative pathogens in these study sites and also
demonstrated their resistance to a few antibiotics. This highlights the need for new antimicrobials that are potent and improved
policy on use of antibiotics.
Antibiotic resistance; ESBLs; Escherichia coli; Klebsiella pneumoniae; Anyigba.