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Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 16, No. 3, 2012, pp. 223-226
Bioline Code: ja12038
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Applied Sciences and Environmental Management, Vol. 16, No. 3, 2012, pp. 223-226

 en Isolation and Screening of Highly Cellulolytic Filamentous Fungi
Khokhar, Ibatsam; Haider, Muhammad Saleem; Mushtaq, Sobia & Mukhtar, Irum

Abstract

A large number of microorganisms are capable of degrading cellulose, only a few of these microorganisms produce significant quantities of enzymes capable of completely hydrolysing cellulose. Fungi are the main cellulase-producing microorganisms. In this purposed study, seventeen fungal species belonging to three genera i.e. Trichoderm check for this species in other resources , Aspergillus check for this species in other resources and Penicillium check for this species in other resources were isolated from different sources, screened and compared for their ability to degrade cellulose. The plate screening assay recommended by International Union of Pure and Applied Chemistry (IUPAC) were used in the investigation. Cellulolytic fungi were evaluated after 7 days for the production of cellulolytic enzymes by staining with 1% Congo red. The diameter of clear zone on fungal plates, gave an approximate indication of cellulase activities. Fungal species were grouped as high and low celluloytic isolates on the basis of cellulase activity using Index of Relative Enzyme Activity (ICMC). Fungal species i.e. Trichoderm harzianum check for this species in other resources , Trichoderm viride check for this species in other resources , Trichoderm koningii check for this species in other resources , Aspergillus japonicus check for this species in other resources , Aspergillus nidulans ver. dentatus check for this species in other resources Penicillium lanosum check for this species in other resources , Penicillium expansum check for this species in other resources and Penicillium oxalicum check for this species in other resources gave the highest cellulase activity. Whereas, Aspergillus flavus check for this species in other resources , Aspergillus raperi check for this species in other resources , Aspergillus acculeatus check for this species in other resources , Aspergillus tamarri check for this species in other resources , Aspergillus niger check for this species in other resources , Aspergillus terreus check for this species in other resources , Aspergillus nidulans, Penicillium citrinum check for this species in other resources and Penicillium simplicissimum check for this species in other resources showed least or no enzyme activity.

Keywords
Cellulose; cellulolytic fungi; Trichoderma, fungal isolates, Congo red

 
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