The isolated compounds from the petroleum spirit, chloroform and methanol
crude leaf extracts of the white specie of the leaf of the
Sesamum Indicum
used by traditional
medicinal practitioners for the management of infecticious diseases were investigated for
in
vitro antimicrobial activity against some organisms. The various extracts afforded
compounds which had significant antimicrobial activities. The antimicrobial screening
showed that all the pure isolates from the different solvent extracts were active against the
organisms,
Staphylococcus aureus
,
Streptococcus pyogenes
,
Salmonella typhi
,
Candida albicans
,
Candida krusei
, and
Candida tropicalis
at various MIC’s and (MBC/MFC)’s. The
pure isolates from methanol and ethyl acetate fractions were all inactive against
Bacillus cereus
,
Corynebacterium ulcerans
, and
Escherichia coli
. Only the pure isolates from
petroleum spirit and chloroform fractions showed activity against
Bacillus cereus,
Corynebacterium ulcerans, and
Escherichia coli at an MIC of 0.5mg/ml and MCB/MFC of
1mg/ml. Chromatographic techniques such as analytical TLC, Purification by dry vacuum
liquid chromatography (DVLC) and Preparative TLC were used.
