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Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 18, No. 4, 2014, pp. 568-574
Bioline Code: ja14075
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Applied Sciences and Environmental Management, Vol. 18, No. 4, 2014, pp. 568-574

 en Evaluation of antimicrobial Activities of isolated compounds from the leaf of the white specie of Sesamum Indicum check for this species in other resources from Benue State, Nigeria
OGWUCHE, CHRISTIANA .E.; AMUPITAN, JOSEPH .O. & NDUKWE, GEORGE .I.

Abstract

The isolated compounds from the petroleum spirit, chloroform and methanol crude leaf extracts of the white specie of the leaf of the Sesamum Indicum check for this species in other resources used by traditional medicinal practitioners for the management of infecticious diseases were investigated for in vitro antimicrobial activity against some organisms. The various extracts afforded compounds which had significant antimicrobial activities. The antimicrobial screening showed that all the pure isolates from the different solvent extracts were active against the organisms, Staphylococcus aureus check for this species in other resources , Streptococcus pyogenes check for this species in other resources , Salmonella typhi check for this species in other resources , Candida albicans check for this species in other resources , Candida krusei check for this species in other resources , and Candida tropicalis check for this species in other resources at various MIC’s and (MBC/MFC)’s. The pure isolates from methanol and ethyl acetate fractions were all inactive against Bacillus cereus check for this species in other resources , Corynebacterium ulcerans check for this species in other resources , and Escherichia coli check for this species in other resources . Only the pure isolates from petroleum spirit and chloroform fractions showed activity against Bacillus cereus, Corynebacterium ulcerans, and Escherichia coli at an MIC of 0.5mg/ml and MCB/MFC of 1mg/ml. Chromatographic techniques such as analytical TLC, Purification by dry vacuum liquid chromatography (DVLC) and Preparative TLC were used.

Keywords
Anti-microbial; Benue; Pathogenic micro-organisms; Sesamum indicum leaves; Dry vacuum; liquid chromatography (DVLC)

 
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