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Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 19, No. 2, 2015, pp. 303-308
Bioline Code: ja15039
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Applied Sciences and Environmental Management, Vol. 19, No. 2, 2015, pp. 303-308

 en In vitro assessment of the antimicrobial activities of leaf and stem extracts of Alchornea cordifolia check for this species in other resources
OWOSENI, ABIMBOLA A.; AYANSINA, AYANTADE D. & AMJO, OLUBUNMI D.

Abstract

In vitro assessment of the antimicrobial effect of leaf and stem extracts of a commonly used plant – Alchornea cordifolia check for this species in other resources was carried out on 10 bacteria and 5 fungi. Five solvents– chloroform, methanol, ethanol, petroleum ether and water were used for extraction. Chloroform extract from leaf and stem resulted in the highest zone of inhibition against the test bacteria (33 – 56mm). Ethanol extracts showed inhibition zone ranging between 10 – 14mm; methanol extract showed inhibition zone of 13 – 21mm only for the leaf extract. Petroleum ether extract of only the stem resulted in inhibition of the test bacteria (10 – 15mm). Leaf and stem water extracts did not result in any zone of inhibition. Only chloroform extracts from both leaf and stem resulted in fungal growth inhibition ranging from 22 – 60mm (leaf) and 14 – 40mm (stem). Staphylococcus haemolyticus check for this species in other resources was most sensitive to leaf chloroform extracts (50mm), followed by E. coli check for this species in other resources (49mm); while Salmonella check for this species in other resources sp. was the most sensitive to stem extracts (56mm). Rhizopus check for this species in other resources sp. was the most sensitive to both chloroform leaf extracts (60mm) and stem extracts (40mm) out of the 5 fungi screened. The highest minimum inhibition concentration (MIC) of 0.125mg/ml was obtained from the leaf chloroform extract on Pseudomonas check for this species in other resources sp. The implication of the findings was discussed.

Keywords
Alchornea cordifolia; plant extracts; antimicrobial; bacteria; fungi

 
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