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Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 19, No. 4, 2015, pp. 611-619
Bioline Code: ja15077
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Applied Sciences and Environmental Management, Vol. 19, No. 4, 2015, pp. 611-619

 en Evaluation of the Phytochemical and Antimicrobial potential of the Leaf Extracts of Bryophyllum pinnatum check for this species in other resources L.and Citrus aurantifolia check for this species in other resources Sw. and their Synergy.
AKINNIBOSUN, F. I. & EDIONWE, O.

Abstract

The aim of this research is to comparatively study the phytochemical and antimicrobial properties of Bryophyllum pinnatum check for this species in other resources and Citrus aurantifolia check for this species in other resources leaf extracts and their synergy. Escherichia coli check for this species in other resources , Staphylococcus aureus check for this species in other resources , Klebsiella pneumoniae check for this species in other resources , and Pseudomonas aeruginosa check for this species in other resources , Aspergillus niger check for this species in other resources , Mucor mucedo check for this species in other resources , Penicillium notatum check for this species in other resources and Candida albicans check for this species in other resources , were used as test organisms and the antimicrobial activity of the extracts was determined by the agar-well diffusion method. Synergistic antibacterial activity ranged from 0.0 ± 0.0 to 2.4 ± 0.6, 11.3 ± 0.9 to 23.5 ± 1.1, 16.7 ± 0.3 to 27.3 ± 0.6 and 8.7 ± 0.9 to 22.7 ± 0.9, for aqueous, ethanol, methanol and acetone extracts respectively. Extracts of C. aurantifolia were more effective against the test organisms than B. pinnatum extracts, except the aqueous extract. Synergistic antifungal activity of the aqueous extract was 0.0 ± 0.0 mm for all the test fungi, the synergistic antifungal activity ranged from 8.7 ± 0.6 mm to 14.0 ± 0.9 mm, 10.0 ± 0.9 mm to 21.7 ± 0.6 mm and 0.0 ± 0.0 mm to 20.0 ± 0.6 mm for the ethanol, methanol and acetone extract respectively. Larger zones of inhibition were observed in the methanol extract of the synergy than the other extracting solvents. The synergy gave higher zones of inhibition neither B. pinnatum extract nor C. aurantifolia extract could give. It was also observed that the extracts compared well with the standard antimicrobial agents used as positive control. The phytochemical analysis of the extract revealed the presence of phytochemical constituents which conferred antimicrobial property on the plants. From the foregoing, the methanol extract of the synergy is considered the most effective in the treatment of infections caused by the test organisms.

Keywords
Bryophyllum pinnatum; Citrus aurantifolia; Synergy; Antimicrobial; Phytochemical constituents

 
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