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Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 20, No. 1, 2016, pp. 103-110
Bioline Code: ja16013
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Applied Sciences and Environmental Management, Vol. 20, No. 1, 2016, pp. 103-110

 en GC-MS Analysis, Antimicrobial and Antioxidant Activities of Extracts of the Aerial Parts of Conyza sumatrensis check for this species in other resources
AIYELAAGBE, OLAPEJU O.; OGUNTOYE, STEPHEN O.; HAMID, ABDULMUMEEN A.; OGUNDARE, ADEBOLA M.; OJO, DAVID B.; AJAO, AJIBOLA & OWOLABI, NURUDEEN O.

Abstract

Phytochemical analyses as well as antimicrobial and antioxidant activities of the extracts of C. sumatrensis check for this species in other resources aerial parts were investigated in this study.
METHODS: The aerial parts of C. sumatrensis were air dried, weighed and exhaustively extracted with hexane, ethyl acetate and methanol successively. The crude extracts were screened for metabolites. These extracts of the plant were evaluated for antimicrobial and antioxidant activities using agar diffusion and DPPH method respectively. The extracts were also analysed using Gas chromatography – Mass spectrometry, and the chromatogram coupled with mass spectra of the compounds were matched with a standard library.
RESULTS: Preliminary phytochemical investigation of crude n-hexane, ethyl acetate and methanol extracts of the aerial parts of Conyza sumatrensis revealed the presence of anthraquinones, flavonoids, terpenoids, phenolics, tannin, glycosides and carbohydrate. All the crude extracts gave a clear zone of inhibition against the growth of the test bacteria ( Staphylococcus aureus check for this species in other resources , Escherichia coli check for this species in other resources , Bacillus subtilis check for this species in other resources , Pseudomona aeruginosa, Salmonella typhi check for this species in other resources , Klebsiellae pneumonae check for this species in other resources ) at moderate to high concentrations, as well as test fungi ( Candida albicans check for this species in other resources , Aspergillus niger check for this species in other resources , penicillium notatum check for this species in other resources and Rhizopus stolonifer check for this species in other resources ) at high concentration. Methanolic extract exhibited significant radical scavenging property with IC50 of 17.08 μg/mL while n-hexane and ethyl acetate extracts showed no significant antioxidant activity. GC-MS of N-hexane extract showed a total number of eleven chemical constituents with α-Farnesene and spathulenol being the most abundance compounds constituting 20.27 and 22.28% of the extract respectively. Ethyl acetate extract revealed thirteen compounds with two most abundant compounds, cis-β-farnesene (16.64 %) and cis-pinane (21.09 %). While methanolic extract affords seventeen compounds with Ephytol being the most abundant compound (19.36 %).

Keywords
Antimicrobial activity; Antioxidant; Conyza sumatrensis; GC-MS analysis; Phytochemicals

 
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