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Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 21, No. 4, 2017, pp. 641-645
Bioline Code: ja17068
Full paper language: English
Document type: Research Article
Document available free of charge

Journal of Applied Sciences and Environmental Management, Vol. 21, No. 4, 2017, pp. 641-645

 en Isolation and Characterization of Hydrocarbon-Degrading Bacteria in Top and Subsoil of selected Mechanic Workshops in Benin City Metropolis, Nigeria
EBAKOTA, OMONIGHO DANIEL; OSARUEME, JOHNSON OSAZEE; GIFT, ONYINYE NWAEZE; ODOLIGIE, IMARHIAGBE & OSAZEE, JOSEPH OSAMUDIAMEN

Abstract

The isolation of hydrocarbon-degrading bacteria in topsoil and subsoil samples of selected mechanic workshops located in the Benin metropolis was carried out. The highest and lowest bacterial counts in topsoil samples were found out to be 2.94 x 106 CFU/g and 2.39 x 106 CFU/g in CENTRAL road and OGBELAKA road automobile workshops respectively. The highest and lowest bacterial counts in subsoil samples were found out to be 1.14 x 106 CFU/g and 1.09 x 106 CFU/g in OGBELAKA r oad and CENTRAL road automobile workshops respectively. The bacterial species isolated were Bacillus check for this species in other resources spp., Pseudomonas check for this species in other resources spp., Staphylococcus check for this species in other resources spp. and Streptococcus check for this species in other resources spp. The isolates were also subjected to hydrocarbon degradation/utilization test where it was observed that Pseudomonas spp utilized the hydrocarbon in the medium more efficiently than the other isolates. The isolates from this study are thus recommended as bacterial species for possible use in bioremediation of hydrocarbons.

Keywords
bioremediation; topsoil; subsoil; hydrocarbon; Pseudomonas spp; isolates.

 
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