Modelling Growth Kinetics of Klebsiella   sp. FIRD 2 on TBT-Resistant Containing Lead

Tributyltin (TBT) is one of the most toxic substances ever deliberately introduced into the marine environment. The high toxicity of TBT has resulted in a wide range of adverse effects on biological systems ranging from bacteria to mammals and from the molecular to the community level. One of the most deleterious effects of TBT is imposex. The growth kinetics of TBT-Resistant Bacterium containing lead was studied. In this study various lead concentrations ranging from 1 to 100 mg/dm³ were used. Seven kinetic models (Teissier, Monod, Yano, Luong, Aiba, Webb, and Haldane,) were investigated and the accuracy of the fitted models were evaluated using statistical analysis such as coefficient of determination, adjusted coefficient of determination (R²) and root mean square (RMSE). Aiba model was fitted to the experimental growth kinetics data and gave a very good fit with an R² of 0.98 and RMSE of 0.0042 respectively. The calculated value for the Aiba constants such as maximal growth rate, half saturation constant and half inhibition constant rate symbolized by μ_max, k_s, and k_i, were 0.038 hr^-1, 0.38s mg/dm³ and 34.38 mg/dm³ respectively. This is the first report of growth kinetics of TBT-Resistant bacterium by Klebsiella sp. FIRD 2 Containing lead.

Keywords
Growth Kinetic models; Klebsiella sp. FIRD 2; lead; TBT-resistant bacteria.